Scientists discover microbes in the Alps and

Biodegradation of plastic at low temperatures can save money and energy

Frontiers

Finding, cultivating, and bioengineering organisms that can digest plastic not only aids in the removal of pollution, but is now also big business. Several microorganisms that can do this have already been found, but when their enzymes that make this possible are applied at an industrial scale, they typically only work at temperatures above 30°C. The heating required means that industrial applications remain costly to date, and aren't carbon-neutral. But there is a possible solution to this problem: finding specialist cold-adapted microbes whose enzymes work at lower temperatures.

Scientists from the Swiss Federal Institute WSL knew where to look for such micro-organisms: at high altitudes in the Alps of their country, or in the polar regions. Their findings are published in Frontiers in Microbiology.

"Here we show that novel microbial taxa obtained from the ‘plastisphere’ of alpine and arctic soils were able to break down biodegradable plastics at 15°C," said first author Dr Joel Rüthi, currently a guest scientist at WSL. "These organisms could help to reduce the costs and environmental burden of an enzymatic recycling process for plastic."

Rüthi and colleagues sampled 19 strains of bacteria and 15 of fungi growing on free-lying or intentionally buried plastic (kept in the ground for one year) in Greenland, Svalbard, and Switzerland. Most of the plastic litter from Svalbard had been collected during the Swiss Arctic Project 2018, where students did fieldwork to witness the effects of climate change at first hand. The soil from Switzerland had been collected on the summit of the Muot da Barba Peider (2,979 m) and in the valley Val Lavirun, both in the canton Graubünden.

The scientists let the isolated microbes grow as single-strain cultures in the laboratory in darkness and at 15°C and used molecular techniques to identify them. The results showed that the bacterial strains belonged to 13 genera in the phyla Actinobacteria and Proteobacteria, and the fungi to 10 genera in the phyla Ascomycota and Mucoromycota.

Surprising results

They then used a suite of assays to screen each strain for its ability to digest sterile samples of non-biodegradable polyethylene (PE) and the biodegradable polyester-polyurethane (PUR) as well as two commercially available biodegradable mixtures of polybutylene adipate terephthalate (PBAT) and polylactic acid (PLA).

None of the strains were able to digest PE, even after 126 days of incubation on these plastics. But 19 (56%) of strains, including 11 fungi and eight bacteria, were able to digest PUR at 15°C, while 14 fungi and three bacteria were able to digest the plastic mixtures of PBAT and PLA. Nuclear Magnetic Resonance (NMR) and a fluorescence-based assay confirmed that these strains were able to chop up the PBAT and PLA polymers into smaller molecules.

"It was very surprising to us that we found that a large fraction of the tested strains was able to degrade at least one of the tested plastics," said Rüthi.

The best performers were two uncharacterized fungal species in the genera Neodevriesia and Lachnellula: these were able to digest all of the tested plastics except PE. The results also showed that the ability to digest plastic depended on the culture medium for most strains, with each strain reacting differently to each of four media tested.

Side-effect of ability to digest plant polymers

How did the ability to digest plastic evolve? Since plastics have only been around since the 1950s, the ability to degrade plastic almost certainly wasn't a trait originally targeted by natural selection.

"Microbes have been shown to produce a wide variety of polymer-degrading enzymes involved in the break-down of plant cell walls. In particular, plant-pathogenic fungi are often reported to biodegrade polyesters, because of their ability to produce cutinases which target plastic polymers due their resemblance to the plant polymer cutin," explained last author Dr Beat Frey, a senior scientist and group leader at WSL.

Challenges remain

Since Rüthi et al. only tested for digestion at 15°C, they don't yet know the optimum temperature at which the enzymes of the successful strains work.

"But we know that most of the tested strains can grow well between 4°C and 20°C with an optimum at around 15°C," said Frey.

"The next big challenge will be to identify the plastic-degrading enzymes produced by the microbial strains and to optimize the process to obtain large amounts of proteins. In addition, further modification of the enzymes might be needed to optimize properties such as protein stability".

Frontiers in Microbiology

10.3389/fmicb.2023.1178474

Experimental study

Not applicable

Discovery of plastic-degrading microbial strains isolated from the alpine and Arctic terrestrial plastisphere

10-May-2023

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Michael Sander and Mattia Cerri declare collaboration with BASF SE on other projects related to polymer biodegradation and Michael Sander for receiving funding for these projects from BASF SE

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